🐈 Cats > People | 🎬 24fps life | 📷 Clicking shutters & pixels 🖥️ Linux is life | 🚀 Sci-Fi & horror geek | 💜 Vegan nerd | 🐍 Python tinkerer | 🔬 Biotech & AI lover ⚡ AI via Stable Diffusion on AMD ROCm – No CUDA, no problem!
#aiart #stablediffusion #animegirl
14.3.2025 00:25#aiart #stablediffusion #animegirl
#aiart #stablediffusion
Inspired by one of my favorite poems from Silent Hill 2:
"For your grace, I do not plead,
For the flames I set free.
Sisters shrieked, and children cried,
No one made it out alive.
I watched them burn, I heard their cries,
I felt a soothing warmth inside.
It felt so good, I cannot lie,
And for my bliss, they had to die."
#aiart #stablediffusion #animegirl
8.3.2025 21:42#aiart #stablediffusion #animegirl
#aiart #stablediffusion #animegirl
26.2.2025 21:34#aiart #stablediffusion #animegirl
#SyntheticBiology #GeneticEngineering #Microbiology #LaboratoryResearch #BScThesis
Hey, just a quick real-life post! This post is all about the experimental workflow in my bachelor's thesis. From genetic modification to analysis, the key steps of the project are shown here. Each picture represents an important part of the process—starting with experimental design and lab work in molecular biology, followed by verification steps like PCR, gel electrophoresis, and HPLC.
The goal? Boosting 2-butanol production in Acetobacterium woodii by swapping the adh4 promoter. Let's dive into the details!"
Pic 1: Draft of my BSc thesis
Pic 2: The molecular biology lab
Pic 3: PCR for the amplification of RHA, LHA, and the promoters
Pic 4: Gel electrophoresis confirming the successful amplification of RHA, LHA, pta, and rso5
Pic 5: E. coli colonies carrying RHA, LHA, and the promoters (pta, rso5)
Pic 6: Gel electrophoresis to verify successful transformation of the promoters into E. coli – pta successful, rso5 failed
Pic 7: Preparing cryo stocks for the spacer2 and spacer2_pta E. coli strains
Pic 8: Midiprep of plasmid DNA from E. coli
Pic 9: White pellet: the extracted plasmid DNA
Pic 10: NanoDrop measurement of the plasmid DNA
Pic 11: Transformation of plasmid DNA into A. woodii under nitrogen conditions to ensure an anaerobic environment.
Pic 12: Gel electrophoresis confirming the successful promoter exchange in A. woodii
Pic 13: Screenshot from CLC Genomics Workbench: The pta promoter was amplified from A. woodii and sequenced, and the new promoter was successfully integrated
Pic 14: Testing ADH expression under the new promoter (Optical Density at 600/660 nm and HPLC)
Pic 15: Preparing high-performance liquid chromatography (HPLC) samples
Pic 16: The HPLC setup
Pic 17: Analysis of chromatograms in Chromeleon
#aiart #stablediffusion #cabin #mysticalforest
24.2.2025 19:40#aiart #stablediffusion #cabin #mysticalforest
#aiart #animegirl #stablediffusion
23.2.2025 22:16#aiart #animegirl #stablediffusion
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22.2.2025 20:11#aiart #stablediffusion #animegirl
#ai #aiart #stablediffusion #frightened #anime #animegirl
21.2.2025 19:32#ai #aiart #stablediffusion #frightened #anime #animegirl
#ai #aiart #animegirl #stablediffusion #cyberpunk
20.2.2025 23:46#ai #aiart #animegirl #stablediffusion #cyberpunk